CCDC86 Knockout cell line (NCI-H1299)

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CCDC86 Knockout cell line (NCI-H1299) Order

Catalog#

YKO-H516

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CCDC86 Knockout cell line (NCI-H1299)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Gene Name

CCDC86

Gene ID

79080

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate

Accession

CVCL_E0ZI(Cellosaurus)

Target region

Knockout region: Exon 1 (based on transcript CCDC86-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NCI-H1299			STR Info (Cell bank) Cell Line:NCI-H1299-Luc2-tdT-2
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	12	12		12	12
D7S820	10	10		10	10
D16S539	12	13		12	13
VWA	16	18		16	18
TH01	6	9.3		6	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	12	12		12	12
D12S391	21	22
FGA	20	20
D2S1338	23	24
D21S11	32.2	32.2
D18S51	16	16
D8S1179	10	13
D3S1358	17	17
D6S1043	11	11
PENTAE	11	11
D19S433	14	14
PENTAD	13	13
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CCDC86 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1299 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CCDC86 gene knockout CCDC86 Knockout cell line (NCI-H1299) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

CCDC86 Knockout cell line (NCI-H1299)

Catalog#

YKO-H516

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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CCDC86 Knockout cell line (NCI-H1299)

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